SYLLABUS FOR APPLIED BIOTECHNOLOGY

SEMESTER VI PAPER 6.1

APPLIED BIOTECHNOLOGY (5 Hrs/week)

Unit – I Environmental Biotechnology: Water pollution: Biotechnological methods for Sewage and Waste water treatment - Primary treatment - Secondary treatment ( Anaerobic digestion process and anaerobic filter) - Tertiary treatment (Ion exchange method) Bioremediation: Definition -types, Microbial degradation of selected Xenobiotics (Hydrocarbon, Pesticides). Role of genetically engineered micro organisms in bioremediation (Plasmid transfer, Super bug). Biomining, Bioleaching and Biofuels: An overview of the role of microbial technology and biominng, bioleachng and biofuel.

Unit –II Agricultural Biotechnology: Basic technology for plant tissue culture (Callus and Explants culture) Production and application of single cell protein Protoplast Fusion: Somatic hybridization and micropropagation techniques and their applications. Genetic manipulation of 'nif' gene and 'nod' gene of nitrogen fixation. Aquacultue Technology: Application of Biotechnological tools (ELISA & PCR) for disease diagnosis.

 Unit – III Bioprocess / Fermentation Technology: Definition, Products of Commercial importance from Bio process technology. Bioreactors: Principle, Design of conventional and Advanced types (Continuous stirred tank Bioreactors CSTB and Airlift bioreactors).. Metabolite production: Primary metabolites - Ethanol production

secondary metabolites - Penicillin - Enzyme production - galactosidase. Biotransformation: Definition, principle and biotransformation of ethanol

Unit – IV Enzyme Technology: Enzymes definition, nomenclature and properties.Enzyme 

production: Microbial organisms and enzyme production Commercial production of microbial enzymes - technique - industrial application of microbial enzymes - enzyme

immobilization and their application. Enzyme Biosensor: Principle and types of biosensor - Applications of biosensor

Unit – V Human genome project: Introduction, objectives, principle, mapping methods and major contributions of Human genome project. Gene therapy: Introduction, types of gene therapy, vectors used in gene therapy (viral vector) and gene therapy for cancer. DNA applications: DNA probe - methods and mechanism - - DNA finger printing techniques and Application in forensic medicine. DNA vaccines, Bio-weapons.

 Reference books:

1. B.D Singh, ―Biotechnology,‖ Kalyani Publishers, No.1 Mahalakshmi street, T.Nagar, Chennai – 600 017

 2. C.Ratledge & B. Kristiansen ―Basic Biotechnology‖ Cambridge University Press.

3. Prof. V. Kumaresan, ―Animal Biotechnology‖, Saras Publication, 114/35G A.R.P Camp Road, Periavilai, Kattar P.O., Nagercoil, Kanyakumari -629 002.

4. Dubey R.C ―A tex book of Biotechnology‖ S. Chand & Co., Ltd., New Delhi.

PRACTICALS

1. Estimation of BOD in sewage-Demonstration

 2. Protoplast preparation and Fusion-Demonstration

3. Estimation of oxygen or carbon dioxide in any effluent/sewage-Individual

 4. MODELS, CHARTS, PHOTOS, AND SLIDES Anaerobic digestor-

Filter,biosensor,callus,explants,micropropagation,protoplast fusion,fermentor,enzyme(structure),RecombinantDNA,HumanGenorneSequence, Penicillin(Structure),Rhizobium,Blue Green Algae(Nostoc) Azolla